Crotoxin effects on Torpedo californica cholinergic excitable vesicles and the role of its phospholiphase A activity
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The phospholipolytic neurotoxin from Crotalus durissus terrificus, crotoxin, is able to produce a dose- and time-dependent block of carbachol-stimulated /sup 22/Na efflux from pre-loaded Torpedo californica excitable vesicles. The blocking activity is dependent on calcium and is abolished by chemical modification with p-bromophenacyl bromide. The isolated basic subunit, crotoxin B, produces an identical block, whereas the isolated acidic subunit, crotoxin A, has no detectable effect. Neither crotoxin nor crotoxin B antagonizes the binding of (/sup 125/I)-..cap alpha..-bungarotoxin to purified acetylcholine receptor, although, at high concentrations, they antagonize its binding to acetylcholine receptor-rich membrane fragments. Certain phospholipase A/sub 2/ enzymes and the fatty acid products of their digestion can mimic the crotoxin action. It is therefore suggested that, although considered a pre-synaptic neurotoxin, crotoxin can have in vitro post-synaptic effects, possibly mediated by its endogeneous phospholipase A/sub 2/ activity.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 5099403
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 82:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALI METAL ISOTOPES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIGENS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EEL
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FISHES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASES
MATERIALS
MEDICINE
NEUROLOGY
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SODIUM 22
SODIUM ISOTOPES
SYNERGISM
TIME DEPENDENCE
TOXIC MATERIALS
TOXINS
VENOMS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALI METAL ISOTOPES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ANTIGENS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EEL
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FISHES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASES
MATERIALS
MEDICINE
NEUROLOGY
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SODIUM 22
SODIUM ISOTOPES
SYNERGISM
TIME DEPENDENCE
TOXIC MATERIALS
TOXINS
VENOMS
VERTEBRATES
YEARS LIVING RADIOISOTOPES