Response of CHO cell proliferation and histone phosphorylation to sodium arsenite

Journal Article · Tue Jan 01 04:00:00 EST 1980 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398009529833· OSTI ID:5098808

Gurley, L R; Walters, R A; Jett, J H; Tobey, R A

Because of the causal association between arsenic and cancer in humans, studies were initiated to determine whether low levels of arsenic affect cell proliferation and chromatin constituents. Cultured CHO cells were treated with As(III) or As(V) in the form of sodium arsenite (NaAsO/sub 2/) or sodium arsenate (Na/sub 2/HAsO/sub 4/), respectively. Arsenite was 10 times more toxic than arsenate. At lower concentrations, 10 ..mu..M NaAsO/sub 2/ induced culture growth kinetics that were radiomimetic. During this division delay, histone Hl phosphorylation was reduced to 40% of the control level. It recovered to the control level just before recovery of cell division. Histone H2A phosphorylation was stimulated in the middle of the delay period, followed by stimulation of H3 phosphorylation just before recovery of cell division. ATP pool levels were unaffected at these low arsenite concentrations. The DNA synthesis rate was also reduced during division delay, but it did not recover immediately before resumption of cell division. Flow microfluorometric analysis showed that cell cycle progression was inhibited in G/sub 1/, S, and G/sub 2/ during the division delay period. On recovery of cell division, cells in S and G/sub 2/ regained the capacity to resume cycle traverse earlier than did cells in G/sub 1/. Cell survival studies indicated that 89% of the cells survived 24-h treatment with 10 ..mu..M NaAsO/sub 2/.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 5098808
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 6:1; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ARSENIC COMPOUNDS
ATP
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CELL CYCLE
CELL DIVISION
CELL PROLIFERATION
CHEMICAL REACTIONS
CHROMATIN
DISEASES
DNA
GENETIC EFFECTS
HISTONES
INHIBITION
KINETICS
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
SYNTHESIS
TOXICITY