Influence of pH extremes on sporulation and ultrastructure of sarcina ventriculi
- Michigan State Univ., East Lansing (USA)
- Michigan Biotechnology Institute, Lansing (USA)
Distinct morphological changes in the ultrastructure of Sarcina ventriculi were observed when cells were grown in medium of constant composition at pH extremes of 3.0 and 8.0. Transmission electron microscopy revealed that at low pH ({le}3.0) the cells formed regular packets and cell division was uniform. When the pH was increased (to {ge}7.0), the cells became larger and cell division resulted in irregular cells that varied in shape and size. Sporulation occurred at high pH (i.e., {ge}8.0). The sporulation cycle followed the conventional sequence of development for refractile endospores, with the appearance of a cortex and multiple wall layers. The spores were resistant to oxygen, lysozyme, or heating at 90{degree}C for 15 min. Spores germinated within the pH range of 4.6 to 7.0.
- DOE Contract Number:
- FG02-87ER13719
- OSTI ID:
- 5098549
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 171:7; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIA
BIOLOGICAL ADAPTATION
HYDROGEN IONS 1 PLUS
BIOLOGICAL EFFECTS
BACTERIAL SPORES
ULTRASTRUCTURAL CHANGES
CATIONS
CHARGED PARTICLES
HYDROGEN IONS
IONS
MICROORGANISMS
MORPHOLOGICAL CHANGES
SPORES
560300* - Chemicals Metabolism & Toxicology