Photoaffinity labeling of the messenger RNA cap-binding protein
Journal Article
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5096717
A photoaffinity derivative of a cap analogue, ..gamma..(/sup 32/P)-(4-(benzoylphenyl)methylamido)-7-methylguanosine 5'-triphosphate, was reacted with the 28-kDa messenger RNA cap-binding protein (CBP), purified by affinity chromatography from rabbit erythrocyte lysate. The predominant labeling was of the 28-kDa species. No labeling occurred without photoillumination or in the presence of 250 ..mu..M m/sup 7/GTP. In addition CBP, two polypeptides of 19 and 17kDa, present in the affinity-purified preparation, were specifically labeled. Another sample tested was the eIF-3 region of a sucrose gradient fractionation, in 100 mM KCl, of rabbit reticulocyte high salt ribosomal wash. The only polypeptide labeled was the 28-kDa CBP. Labeling was more intense than with an equivalent amount of affinity-purified CBP, suggesting the presence of stabilizing or stimulatory factors in the eIF-3 preparation.
- Research Organization:
- Univ. of Kentucky, Lexington
- OSTI ID:
- 5096717
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Conference
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Sat Jan 04 23:00:00 EST 1986
· J. Biol. Chem.; (United States)
·
OSTI ID:5150816
Phenyl azide substituted and benzophenone-substituted phosphonamides of 7-methylguanosine 5 prime -triphosphate as photoaffinity probes for protein synthesis initiation factor eIF-4E and a proteolytic fragment containing the cap-binding site
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Tue Jun 12 00:00:00 EDT 1990
· Biochemistry; (United States)
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OSTI ID:5229493
Photoaffinity labeling of the cap-binding protein complex with ATP/dATP. Differential labeling of free eukaryotic initiation factor 4A and the eukaryotic initiation factor 4A component of the cap-binding protein complex with (alpha-TSP)ATP/dATP
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Mon Nov 04 23:00:00 EST 1985
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OSTI ID:6086294
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LIGHT NUCLEI
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
TRACER TECHNIQUES