Hydrocortisone effect of arylsulfatase A in primary mouse brain cell cultures
The primary goal of this study was to study the mechanism of action of hydrocortisone (HC) on arylsulfatase A (ASA) in primary cultures of cells that were dissociated from the brains of embryonic mice. Cells were cultured in a defined medium in the absence or in the presence of 3 ..mu..M HC. The specific activity of ASA in nontreated cells was 1.297 U/mg (U = ..mu..mol/hr) while the value for the HC-treated cells was 0.783 U/mg. The authors data shows that HC inhibits ASA activity in these cultures cells (p < 0.001). The determination of the ASA enzyme activity was assayed primarily with the artificial substrate p-nitrocatechol sulfate. However, the natural substrate (cerebroside /sup 35/S-sulfate) also as active and correlated linearly with the activity of p-nitrocatechol sulfate. Purified ASA was isolated from calf brains and used to generate an antibody (Ab) against ASA. The specificity of the Ab for the ASA protein of cell cultures was tested in Ouchterlony double immunodiffusion studies. The Ab was used in a competitive enzyme-linked immunosorbent assay to quantify the number of ASA molecules in the cell extracts from the embryonic mouse cell cultures. Preliminary data suggest that HC decreases the number of ASA molecules.
- Research Organization:
- Temple Univ. School of Medicine, Philadelphia, PA
- OSTI ID:
- 5096667
- Report Number(s):
- CONF-8606151-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Comparisons of the effects of TCDD and hydrocortisone on growth factor expression provide insight into their interaction in the embryonic mouse palate
Developmental onset of mixed-function oxidase activity in preimplantation mouse embryos
Related Subjects
HYDROCORTISONE
BIOLOGICAL EFFECTS
HYDROLASES
ENZYME ACTIVITY
BRAIN
CELL CULTURES
EMBRYOS
IMMUNE REACTIONS
INHIBITION
MICE
MOLECULES
SULFATES
SULFUR 35
TRACER TECHNIQUES
ADRENAL HORMONES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PREGNANES
RADIOISOTOPES
RODENTS
STEROID HORMONES
STEROIDS
SULFUR COMPOUNDS
SULFUR ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques