Biotransformation of 1-nitropyrene to 1-aminopyrene and N-formyl-1-aminopyrene by the human intestinal microbiota
The nitropolycyclic aromatic hydrocarbon 1-nitropyrene (1-NP) is an environmental pollutant, a potent bacterial and mammalian mutagen, and a carcinogen. The metabolism of 1-NP by the human intestinal microbiota was studied using a semicontinuous culture system that simulates the colonic lumen. (/sup 3/H)-1-Nitropyrene was metabolized by the intestinal microbiota to 1-aminopyrene (1-AP) and N-formyl-1-aminopyrene (FAP) as determined by high-performance liquid chromatography (HPLC) and mass spectrometry. Twenty-four hours after the addition of (/sup 3/H)-1-NP, the formylated compound and 1-AP accounted for 20 and 80% of the total metabolism respectively. This percentage increased to 66% for FAP after 24 h following 10 d of chronic exposure to unlabeled 1-NP, suggesting metabolic adaptation to 1-NP by the microbiota. Both 1-AP and FAP have been shown to be nonmutagenic towards Salmonella typhimurium TA98, which indicates that the intestinal microflora may potentially detoxify 1-NP.
- Research Organization:
- Univ. of Alabama, Birmingham
- OSTI ID:
- 5095369
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 18:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
POLYCYCLIC NITRO COMPOUNDS
METABOLISM
BIOLOGICAL ADAPTATION
CHRONIC EXPOSURE
DETOXIFICATION
EXPERIMENTAL DATA
INTESTINES
MAN
MICROORGANISMS
POLYCYCLIC AROMATIC HYDROCARBONS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AROMATICS
BODY
DATA
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HYDROCARBONS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NITRO COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PRIMATES
VERTEBRATES
550501* - Metabolism- Tracer Techniques