Degradation of pyridine by Micrococcus luteus isolated from soil
An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or hydroxy-, chloro-, amino-, or methylpyridines. Cell extracts of M. luteus could not degrade pyridine, 2-, 3-, or 4-hydroxypyridines or 2,3-dihydroxypyridine, regardless of added cofactors or cell particulate fraction. The organism had a NAD-linked succinate-semialdehyde dehydrogenase which was induced by pyridine. Cell extracts of M. luteus had constitutive amidase activity, and washed cells degraded formate and formamide without a lag. These data are consistent with a previously reported pathway for pyridine metabolism by species of Bacillus, Brevibacterium, and Corynebacterium. Cells of M. luteus were permeable to pyridinecarboxylic acids, monohydroxypyridines, 2,3-dihydroxypyridine, and monoamino- and methylpyridines. The results provide new evidence that the metabolism of pyridine by microorganisms does not require initial hydroxylation of the ring and that permeability barriers do not account for the extremely limited range of substrate isomers used by pyridine degraders.
- Research Organization:
- Purdue Univ., West Lafayette, IN
- OSTI ID:
- 5093357
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 51:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MICROCOCCUS
GROWTH
PYRIDINE
BIODEGRADATION
BIOLOGICAL PATHWAYS
METABOLISM
PERMEABILITY
SOILS
SPECIFICITY
SUBSTRATES
AZINES
BACTERIA
CHEMICAL REACTIONS
DECOMPOSITION
HETEROCYCLIC COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
550700* - Microbiology
550500 - Metabolism