Regional distribution of immunoreactive endothelin in rats

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Peptides (Fayetteville, New York); (USA)

DOI:https://doi.org/10.1016/0196-9781(89)90117-4· OSTI ID:5085530

Yoshimi, H; Hirata, Y; Fukuda, Y; Kawano, Y; Emori, T; Kuramochi, M; Omae, T; Marumo, F ^[1]

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1. National Cardiovascular Center Research Institute and Hospital, Osaka (Japan)

By use of a specific radioimmunoassay for endothelin (ET), the regional distribution of ET-like immunoreactivity (LI) was studied in rats. The antiserum used cross-reacted equally with synthetic porcine and rat ET. Significant amounts of ET-LI are detectable not only in aorta, but also in kidney, lung, heart, liver and central nervous system. Gel chromatography of the tissue extracts revealed size heterogeneity of ET-LI; one major component eluting close to, but slightly larger than standard rat ET and the other minor component with a larger molecular weight. These data indicate that ET-LI is widely distributed throughout the various rat tissues, suggesting its possible involvement in a variety of organ functions.

• OSTI ID: 5085530
• Journal Information: Peptides (Fayetteville, New York); (USA), Journal Name: Peptides (Fayetteville, New York); (USA) Vol. 10:4; ISSN PPTDD; ISSN 0196-9781
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISTRIBUTION
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RODENTS
SEPARATION PROCESSES
SWINE
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES