Isolation and characterization of a novel gene close to the human phosphoinositide-specific phospholipase C {beta}3 gene on chromosomal region 11q13

Journal Article · Thu Feb 01 00:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0063· OSTI ID:508340

Lagercrantz, J; Carson, E; Larsson, C ^[1]

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1. Karolinska Hospital, Stockholm (Sweden); and others

We describe the isolation, characterization, and genomic structure of a gene, Phospholipase C {beta}3 Neighboring gene (PNG), located on chromosome 11q13. The cDNA was isolated using a cosmid that also contains the phospholipase C{beta}3 gene (PLCB3). PNG does not have any marked similarity to other known genes on the DNA level. However, analysis of hybridization to a panel of somatic cell hybrids indicates the existence of related sequences on chromosomes 2, 4, 7, and 22. PNG showed expression of a 1-kb message in multiple tissues. The predicted protein is 199 amino acids. The gene spans approximately 2.5 kb, divided into four exons and three introns. It is located 4.4 kb upstream of PLCB3, with the 5{prime} ends facing each other. The intergenic region has been completely sequenced, revealing separate CpG islands at both ends of this region. The islands are separated by a stretch of 2 kb, characterized by periodic alteration of the GC content. The 5{prime}-flanking region of PNG does not contain TATA or CCAAT, suggesting a housekeeping promoter structure. 22 refs., 4 figs., 1 tab.

• OSTI ID: 508340
• Journal Information: Genomics, Vol. 31, Issue 3; Other Information: PBD: 1 Feb 1996
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
GENES
STRUCTURE-ACTIVITY RELATIONSHIPS
GENETIC MAPPING
GENE REGULATION
DNA SEQUENCING
TISSUE DISTRIBUTION
SIZE
TRANSCRIPTION
HUMAN CHROMOSOMES
HEREDITARY DISEASES
GENETICS
DNA HYBRIDIZATION
COSMIDS
SOMATIC CELLS
AMINO ACIDS
POLYMERASE CHAIN REACTION
PROBES
NUCLEOTIDES
CONTIGS
ENDOCRINE DISEASES