Sequences more than 500 base pairs upstream of the human U3 small nuclear RNA gene stimulate the synthesis of U3 RNA in frog oocytes

Journal Article · Tue Jun 04 04:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00236a016· OSTI ID:5081240

Suh, D; Reddy, R ^[1]; Wright, D ^[2]

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1. Baylor Coll. of Medicine, Houston, TX (United States)
2. Texas Medical Center, Houston (United States)

Small nuclear RNA (snRNA) genes contain strong promoters capable of initiating transcription once every 4 s. Studies on the human U1 snRNA gene, carried out in other laboratories, showed that sequences within 400 bp of the 5' flanking region are sufficient for maximal levels of transcription both in vivo and in frog oocytes (reviewed in Dahlberg and Lund (1988)). The authors studied the expression of a human U3 snRNA gene by injecting 5' deletion mutants into frog oocytes. The results show that sequences more than 500 bp upstream of the U3 snRNA gene have a 2-3-fold stimulatory effect on the U3 snRNA synthesis. These results indicate that the human U3 snRNA gene is different from human U1 snRNA gene in containing regulatory elements more than 500 bp upstream. The U3 snRNA gene upstream sequences contain an AluI homologous sequence in the {minus}1,200 region; these AluI sequences were transcribed in vitro and in frog oocytes but were not detectable in Hela cells.

• OSTI ID: 5081240
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:22; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
CELL CONSTITUENTS
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
FROGS
GENES
GERM CELLS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OOCYTES
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROMOTERS
RADIOISOTOPES
RNA
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES