Characterization of the testicular cell types present in the rat by in vivo 31P magnetic resonance spectroscopy

Journal Article · Mon Jul 01 04:00:00 EDT 1991 · Biology of Reproduction; (United States)

DOI:https://doi.org/10.1095/biolreprod45.1.122· OSTI ID:5080589

van der Grond, J; Van Pelt, A M; van Echteld, C J; Dijkstra, G; Grootegoed, J A; de Rooij, D G; Mali, W P ^[1]

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1. Department of Radiodiagnosis, University of Utrecht (Netherlands)

Testes of vitamin A-deficient Wistar rats before and after vitamin A replacement, of rats irradiated in utero, and of control rats were investigated by in vivo 31P magnetic resonance (MR) spectroscopy. The testicular phosphomonoester/ATP (PM/ATP) ratio ranged from 0.79 {plus minus} 0.05 for testes that contained only interstitial tissue and Sertoli cells to 1.64 {plus minus} 0.04 for testes in which spermatocytes were the most advanced cell types present. When new generations of spermatids entered the seminiferous epithelium, this ratio decreased. The testicular phosphodiester/ATP (PD/ATP) ratio amounted to 0.16 {plus minus} 0.06 for testes in which Sertoli cells, spermatogonia, or spermatocytes were the most advanced cell type present. When new generations of spermatids entered the seminiferous epithelium, the PD/ATP ratio rapidly increased and finally reached a value of 0.71 {plus minus} 0.06 for fully developed testes. Taken together, specific patterns of the PM/ATP ratio, the PD/ATP ratio, and pH were obtained that were correlated to the presence of spermatogonia, spermatocytes, round spermatids, and elongated spermatids or to the absence of spermatogenic cells. Hence, a good impression of the status of the seminiferous epithelium in the rat can be obtained by in vivo 31P MR spectroscopy.

• OSTI ID: 5080589
• Journal Information: Biology of Reproduction; (United States), Journal Name: Biology of Reproduction; (United States) Vol. 45:1; ISSN 0006-3363; ISSN BIREB
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BODY
CHEMICAL COMPOSITION
ENZYMES
ESTERASES
GAMETES
GAMETOGENESIS
GERM CELLS
GONADS
HYDROLASES
IN VIVO
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MALE GENITALS
MAMMALS
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEOTIDES
NUTRITIONAL DEFICIENCY
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
RATS
RESONANCE
RODENTS
SPECTRA
SPERMATOGENESIS
SPERMATOZOA
STABLE ISOTOPES
TESTES
TRACER TECHNIQUES
VERTEBRATES
VITAMIN A
VITAMINS