Distribution and binding of (/sup 14/C)acrylamide to macromolecules in SENCAR and BALB/c mice following oral and topical administration

Journal Article · Sun Jun 30 04:00:00 EDT 1985 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(85)90352-7· OSTI ID:5079904

Carlson, G P; Weaver, P M

To determine if differences in acrylamide distribution or its binding to DNA could be responsible for the reported higher incidence of skin papillomas observed after oral administration compared to topical application, (/sup 14/C)acrylamide was administered by topical application and oral intubation to male SENCAR and BALB/mice. Portions of lung, liver, stomach, testes, and skin were removed, and /sup 14/C was measured at 15 min, 30 min, 1, 6, 12, 24, and 48 hr. Binding to DNA, RNA, and protein was measured at 6 and 48 hr. Following oral administration, few strain differences in distribution or binding were noted. After topical application, SENCAR mice generally showed higher tissue concentrations than did the BALB/c mice at the early time periods but not at the later ones. Comparing the two routes, comparable concentrations were observed in all tissues except the skin where the amount of (/sup 14/C)acrylamide after topical application was approximately 100 times that observed after oral administration. At 48 hr, binding to DNA was sevenfold higher after topical than after oral administration. The effect of route on papilloma formation cannot be explained, therefore, on the basis of either a difference in distribution or binding to DNA in the target organ. The binding of acrylamide to DNA in skin was similar in both SENCAR and BALB/c mice indicating that the much greater susceptibility of the SENCAR mice to tumorigenesis cannot be explained simply on the basis of distribution or macromolecular binding.

• OSTI ID: 5079904
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Journal Name: Toxicol. Appl. Pharmacol.; (United States) Vol. 2; ISSN TXAPA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACRYLAMIDE
AMIDES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
DNA
GASTROINTESTINAL TRACT
GLANDS
GONADS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
LUNGS
MALE GENITALS
MAMMALS
MICE
MOLECULES
NEOPLASMS
NUCLEIC ACIDS
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RESPIRATORY SYSTEM
RNA
RODENTS
SKIN
STOMACH
TESTES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES