Crystal structure of interleukin 8: Symbiosis of NMR and crystallography

Journal Article · Tue Jan 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.2.502· OSTI ID:5076084

Baldwin, E T; Weber, I T; Charles, R; Xuan, Jiancheng; Matsushima, Kouji; Wlodawer, A ^[1]; Appella, E; Clore, G M; Gronenborn, A M ^[2]; Yamada, Masaki ^[3]; Edwards, B F.P. ^[4]

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1. National Cancer Inst., Frederick, MD (United States)
2. National Inst. of Health, Bethesda, MD (United States)
3. Dainippon Pharmaceutical Co., Ltd., Osaka (Japan)
4. Wayne State Univ. School of Medicine, Detroit, MI (United States)

The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 {angstrom} x-ray data to an R factor of 0.817. A comparison indicates some potential differences between the structure in solution and in the crystalline state. The analysis also predicts that residues 4 through 9 on the amino terminus and the {beta}-bend, which includes His-33, may be important for receptor binding.

• OSTI ID: 5076084
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:2; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
GROWTH FACTORS
X-RAY DIFFRACTION
LYMPHOKINES
NUCLEAR MAGNETIC RESONANCE
AQUEOUS SOLUTIONS
CRYSTALLOGRAPHY
MAN
MONOCYTES
RECEPTORS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COHERENT SCATTERING
DIFFRACTION
DISPERSIONS
LEUKOCYTES
MAGNETIC RESONANCE
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MIXTURES
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RESONANCE
SCATTERING
SOLUTIONS
VERTEBRATES
550602* - Medicine- External Radiation in Diagnostics- (1980-)