Synergistic effects of micromolar concentrations of Zn/sup 2 +/ and Ca/sup 2 +/ on membrane fusion
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Resonance Energy Transfer between N-(7-nitro-2,1,3 benzoxadiazol-4 yl) phosphatidyl ethanolamine and N-Lissamine-Rhodamine B sulfonyl) phosphatidyl ethanolamine embedded in two different populations of small unilamellar vesicles made of phosphatidyl serine has been used to study the fusion process induced by Zn/sup 2 +/ and Ca/sup 2 +/. Lipid intermixing demonstrating fusion of liposome membranes can already be observed at 125 and 250 ..mu..mol/l of Zn/sup 2 +/. After short time pre-incubations with micromolar concentrations of Zn/sup 2 +/ as low as 150 ..mu..mol/l, Ca/sup 2 +/ induces an instantaneous increase of vesicle fusion. The lipid intermixing induced by micromolar concentrations of Ca/sup 2 +/ (250-500 ..mu..mol/l) could be increased up to 4 times when pre-incubated with 150 or 200 ..mu..mol/l of Zn/sup 2 +/. The effect of 1 mM of Ca/sup 2 +/ alone on lipid intermixing can be mimicked by 150 ..mu..mol/l of Zn/sup 2 +/ followed by 500 ..mu..mol/l of Ca/sup 2 +/. The data demonstrate that Zn/sup 2 +/ and Ca/sup 2 +/ act synergistically to affect cation-induced membrane fusion.
- Research Organization:
- Centre de Recherches, Braine-l'Alleud, Belgium
- OSTI ID:
- 5073386
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 137:1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
CALCIUM COMPOUNDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
DATA
ENERGY TRANSFER
ESTERS
EXPERIMENTAL DATA
HYBRIDIZATION
INFORMATION
IONS
LIPIDS
LIPOSOMES
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PHOSPHOLIPIDS
RESPONSE MODIFYING FACTORS
SYNERGISM
ZINC COMPOUNDS
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
CALCIUM COMPOUNDS
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
DATA
ENERGY TRANSFER
ESTERS
EXPERIMENTAL DATA
HYBRIDIZATION
INFORMATION
IONS
LIPIDS
LIPOSOMES
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PHOSPHOLIPIDS
RESPONSE MODIFYING FACTORS
SYNERGISM
ZINC COMPOUNDS