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Stimulatory activities of a T cell proteoglycan fraction (T-PGF)

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5073340
;
The authors had demonstrated previously that T lymphocytes synthesize and secrete chondroitin sulfate proteoglycan (PG). To analyze whether this PG possessed immunoregulatory activity, mouse T cell hybridomas were created and screened for secretion of /sup 35/S-PG. The PG secreted by high producer lines was purified primarily by anion exchange chromatography. Analysis of isolated material indicated the presence of uronic acid, a major sugar in glycosaminoglycans. SDS-PAGE of radio-iodinated T-PGF revealed two bands at 70 kd and 50 kd, which was confirmed by silver staining. The T-PGF stimulated mouse splenic B cell fractions to proliferate and differentiate into plaque-forming cells; no induction or costimulation (with Con A) of T cells fraction or thymocyte proliferation was detected. The T-PGF stimulated activated (Percoll low density, increased forward light scattering) B cells better than resting populations; induction of resting cells could be enhanced by simultaneous addition of low concentrations of a protein mitogen, STM. Biochemically and functionally, T-PGF co-migrates with PG in the void volume of Sephacryl S-200 columns. The precise nature of the relationship between B cell stimulatory activity and PG is unknown; however, the recent production of monoclonal antibodies against T-PGF should help clarify these questions.
Research Organization:
Guthrie Research Institute, Sayre, PA
OSTI ID:
5073340
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL PROLIFERATION
CHROMATOGRAPHY
CONCANAVALIN
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HEMAGGLUTININS
HYBRIDOMAS
IMMUNE REACTIONS
IODINE ISOTOPES
ION EXCHANGE CHROMATOGRAPHY
ISOTOPE APPLICATIONS
ISOTOPES
LECTINS
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MITOGENS
MONOCARBOXYLIC ACIDS
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
PURIFICATION
RADIOISOTOPES
RODENTS
SECRETION
SEPARATION PROCESSES
SOMATIC CELLS
SPLEEN CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
URONIC ACIDS
VERTEBRATES