Autodecomposition of radiolabeled human growth hormone

Journal Article · Wed Jan 01 04:00:00 EST 1986 · J. Immunoassay; (United States)

DOI:https://doi.org/10.1080/01971528608060462· OSTI ID:5072867

Baumann, G; Amburn, K

Human growth hormone (hGH) was radiolabeled with /sup 125/I, using a gentle lactoperoxidase technique. The stability and decomposition products of this tracer were studied by frequent periodic analysis by Sephadex G-100 chromatography on a long column. Monomeric /sup 125/I-hGH showed an exponential decline, with a half-life of 61 days. The main radioactive degradation product was iodide, which appeared with a fractional appearance rate of 0.01136 per day. Secondary degradation products were a series of radioactive oligomers of hGH, which appeared with an overall fractional rate of 0.00525 per day. The kinetic data obtained should provide guidelines for the shelf-life and repurification schedule of radioiodinated polypeptides.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 5072867
• Journal Information: J. Immunoassay; (United States), Journal Name: J. Immunoassay; (United States) Vol. 3; ISSN JOUID
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALF-LIFE
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOCHEMISTRY
RADIOISOTOPES
RADIOPHARMACEUTICALS
STABILITY
STH