On-column digestion of protein for peptide mapping by capillary zone electrophoresis with laser-induced native fluorescence detection

Journal Article · Fri Oct 15 00:00:00 EDT 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00068a040· OSTI ID:5069899

Chang, H T; Yeung, E S ^[1]

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1. Ames Lab., IA (United States) Iowa State Univ., Ames, IA (United States)

We have developed a novel technique to separate and detect peptide fragments which are digested on the same column. In this procedure, pepsin is used to digest low femtomole amounts of [beta]-lactoglobulin on the column. Then, CZE and LINF are applied to separate and detect the peptide fragments. The advantages of this method are its simplicity, high sensitivity, high selectivity, efficient operation, and high speed. 38 refs., 6 figs., 2 tabs.

• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 5069899
• Journal Information: Analytical Chemistry (Washington); (United States), Vol. 65:20; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
GLOBULINS
DIGESTION
PEPTIDES
QUALITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
PROTEINS
DETECTION
CAPILLARY FLOW
COMPILED DATA
ELECTROPHORESIS
FLUORESCENCE
LASER SPECTROSCOPY
PEPSIN
ACID PROTEINASES
CHEMICAL ANALYSIS
DATA
ENZYMES
FLUID FLOW
HYDROLASES
INFORMATION
LUMINESCENCE
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
SPECTROSCOPY
400100* - Analytical & Separations Chemistry
550200 - Biochemistry