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Title: Biodegradation and bioconversions of coals by fungi. Quarterly progress report No. 4, July 1-September 30, 1986

Technical Report ·
OSTI ID:5069792

We have continued the screening of 11 more new lignite-solubilizing isolates tested for activity on the three different lignites as described in previous reports. We have worked on the identification of several strains, especially those which are most active, and have narrowed IDs down to possible genera. Samples were collected in Colorado of weathered and unweathered subbituminous coals of two grades, and a lignite which is different from any already tested. We have several goals in mind: to isolate subbituminous-solubilizing strains from the subbituminous coals; to test our lignite-solubilizing strains on the subbituminous; and to see if we can isolate one or more strains which will solubilize bituminous coals. We have tested several of our lignite-solubilizers on the Colorado subbituminous, and now have good and rapid solubilization on the weathered samples of one coal by some, but not all strains tested. We are screening all isolates to assess their abilities to solubilize lignite on the surface of the mycelial mat. We have tested over 20 strains. We have identified a few strains which, for various reasons of culture habit, ease of handling, and rapidity and degree of solubilization, seem to be good organisms to apply to production of liquid product for further study. We have used a qualitative assay (the syringaldazine spot color test) for laccase and peroxidase activity on both lignite-solubilizers and several strains which will not solubilize lignite. Of the over 50 strains tested, only 6 gave positive tests for laccase, and each of these is an active lignite-solubilizer. Yet about 35 lignite-solubilizers tested did not test positive for laccase, and of these, one tested positive for peroxidase, and a different one gave evidence of catalase. 3 refs., 3 tabs.

Research Organization:
Mississippi Univ., University (USA). Dept. of Biology
DOE Contract Number:
FG22-85PC80913
OSTI ID:
5069792
Report Number(s):
DOE/PC/80913-4; ON: DE87000916
Country of Publication:
United States
Language:
English