Varying levels of radioprotection from the effects of JANUS neutrons in repair-deficient xrs-5 hamster cells treated with azacytidine
- Northern Illinois Univ., DeKalb, IL (United States)
- Univ. of Chicago, IL (United States)
A series of cell lines have been generated from the radiation-sensitive Chinese hamster ovary line xrs-5 by treatment with azacytidine. Several of these lines have been shown to be resistant to {gamma} radiation. Survival curves have been generated for several of these lines and the parental lines after exposure to 0 to 5 Gy of JANUS neutrons in the presence or absence of a 30-min pretreatment with the aminothiol radioprotector WR-1065. These studies were performed to determine whether the parental xrs-5 cell line was radioresistant to exposure to JANUS neutrons and whether reversion to a neutron-resistant phenotype correlated with recovery of aminothiol radioprotection. Exposure to 4 mM WR-1065 enhanced survival after exposure to neutron radiation for most {open_quotes}revertant{close_quotes} lines, although the increase in survival varied. The xrs-5 cell line was sensitive to JANUS neutrons and showed no protection by WR-1065. These data indicate that xrs-5 cells are also sensitive to neutron radiation, that azacytidine-induced revertants for {gamma}-ray survival demonstrate the wild-type phenotype for survival after neutron exposure, and that the gene product that is defective is responsible for repairing only a small portion of neutron-induced damage. 18 refs., 1 fig., 1 tab.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL
- DOE Contract Number:
- W-31109-ENG-38
- OSTI ID:
- 50635
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 3 Vol. 140; ISSN RAREAE; ISSN 0033-7587
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inhibition of topoisomerase II activity in repair-proficient CHO K1 cells by 2-[(aminopropyl)amino]ethanethiol (WR-1065)
Inhibition of topoisomerase II{alpha} activity in CHO K1 cells by 2-[(aminopropyl)amino]ethanethiol (WR-1065)