Assessment of polyamines in the control of cell growth and differentiation in cultured Friend erythroleukemia cells
Conference
·
OSTI ID:5062652
Treatment of cultures of Friend murine erythroleukemia (FL) cells with ..cap alpha..-methylornithine (MO), an inhibitor of polyamine biosynthesis, caused increased ornithine decarboxylase (ODC) activity and decreased levels of putrescine and spermidine. ..cap alpha..-Methylornithine also inhibited cell growth and the dimethylsulfoxide (DMSO)-induced terminal differentiation. Addition of putrescine abrogated these effects. 12-O-Tetradecanoylphorbol-13-acetate (TPA), a potent inhibitor of DMSO-induced differentiation in these cells, did not itself effect ornithine decarboxylase activity. Neither MO nor TPA counteracted the other's action on cell growth and differentiation. These results suggest that: (1) alteration of polyamine levels, but not necessarily changes in ODC activity, may be involved in regulation of growth and differentiation of FL cells and (2) the effect of TPA on the inhibition of DMSO-induced differentiation in FL cells may not be directly mediated via polyamine biosynthesis.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5062652
- Report Number(s):
- CONF-7808127-1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Polyamines and meristematic activity in Zea mays roots
Intracellular polyamine biosynthesis is required for optimal cytolytic T lymphocyte (CTL) induction
Novel agmatine analogue, {gamma}-guanidinooxypropylamine (GAPA) efficiently inhibits proliferation of Leishmania donovani by depletion of intracellular polyamine levels
Journal Article
·
Fri Mar 31 23:00:00 EST 1989
· Plant Physiology, Supplement; (USA)
·
OSTI ID:6611117
Intracellular polyamine biosynthesis is required for optimal cytolytic T lymphocyte (CTL) induction
Conference
·
Fri Feb 28 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5417384
Novel agmatine analogue, {gamma}-guanidinooxypropylamine (GAPA) efficiently inhibits proliferation of Leishmania donovani by depletion of intracellular polyamine levels
Journal Article
·
Fri Oct 10 00:00:00 EDT 2008
· Biochemical and Biophysical Research Communications
·
OSTI ID:21143897
Related Subjects
550200* -- Biochemistry
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACID ESTERS
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
CELL PROLIFERATION
DECARBOXYLASE
DISEASES
ENZYME ACTIVITY
ESTERS
HEMIC DISEASES
INHIBITION
LEUKEMIA
MAMMALS
NEOPLASMS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PUTRESCINE
RATS
RODENTS
SPERMIDINE
SYNTHESIS
TUMOR CELLS
VERTEBRATES
550300 -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOXYLIC ACID ESTERS
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
CELL PROLIFERATION
DECARBOXYLASE
DISEASES
ENZYME ACTIVITY
ESTERS
HEMIC DISEASES
INHIBITION
LEUKEMIA
MAMMALS
NEOPLASMS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PUTRESCINE
RATS
RODENTS
SPERMIDINE
SYNTHESIS
TUMOR CELLS
VERTEBRATES