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Reaction with DNA and mutagenic specificity of syn-benzo[g]chrysene 11,12-dihydrodiol 13,14-epoxide

Journal Article · · Chemical Research in Toxicology
; ; ; ;  [1]; ;  [2];  [3]
  1. ABL-Basic Research Program, Frederick, MD (United States)
  2. Ben May Institute, Chicago, IL (United States)
  3. DynCorp., Inc., Frederick, MD (United States)
The spectroscopic characterization of purine deoxyribonucleoside adducts derived from the fjord-region syn-benzo[g]chrysene 11,12-dihydrodiol 13,14-epoxide and the mutagenic specificity of the latter compound for the supF gene in the pSP189 shuttle vector are described. This dihydrodiol epoxide preferentially forms adducts with deoxyadenosine residues in DNA and is preferentially opened trans in reactions with DNA or with deoxyribonucleotides. In common with other fjord-region syn-dihydrodiol epoxides, the most frequently observed mutational changes were A {yields} T and G {yields} T changes. This hydrocarbon dihydrodiol epoxide is structurally similar to syn-benzo[c]phenanthrene 3,4-dihydrodiol 1,2-epoxide but has an additional benzene ring annelated distant from the reaction center. As anticipated, there were some common features in the chemistry and mutagenicities of these two compounds, but there were also substantive differences which indicate factors of importance in controlling reactions of these kinds of compounds with DNA. 38 refs., 11 figs., 2 tabs.
Sponsoring Organization:
USDOE
OSTI ID:
50622
Journal Information:
Chemical Research in Toxicology, Journal Name: Chemical Research in Toxicology Journal Issue: 3 Vol. 7; ISSN 0893-228X; ISSN CRTOEC
Country of Publication:
United States
Language:
English