Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

EGF-induced mitogenesis in proximal tubular cells: Potentiation by angiotensin II

Journal Article · · American Journal of Physiology; (USA)
OSTI ID:5059985
; ; ; ; ; ;  [1]
  1. Univ. of California, Los Angeles (USA)
+ Show Author Affiliations
The mitogenic effect of epidermal growth factor (EGF) and the characteristics of EGF binding were studied on primary cultures of rabbit proximal tubular cells. EGF was found to be a potent mitogen and stimulated DNA synthesis 18-fold above the level observed in quiescent cells. Using {sup 125}I-EGF as a ligand, two classes of specific EGF receptors were identified on the proximal tubular cell in culture, i.e., a high-affinity receptor with a dissociation constant (K{sub d}) of 0.3 nM and maximal binding (B{sub max}) of 1.7 {times} 10{sup 4} receptors/cell and a low-affinity receptor with a K{sub d} of 1.9 nM and B{sub max} of 5.3 {times} 10{sup 4} receptors/cell. Because angiotensin II (ANG II) appeared to possess many properties common to growth factors, the authors also examined the interaction of ANG II and EGF on these cells. ANG II was not mitogenic, but it potentiated the mitogenic effect of EGF with a maximal effect at 10{sup {minus}9} M. The dose-response curve of EGF-induced mitogenesis was shifted to the left in the presence of 10{sup {minus}9} M ANG II. These studies demonstrate that ANG II potentiates EGF-induced mitogenesis at one or more postreceptor steps that may include small changes in cytosolic Ca{sup 2+} concentration.
OSTI ID:
5059985
Journal Information:
American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:2; ISSN 0002-9513; ISSN AJPHA
Country of Publication:
United States
Language:
English

Similar Records

Angiotensin II binding to cultured bovine adrenal chromaffin cells: identification of angiotensin II receptors
Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6832193
Angiotensin II receptors in amphibian kidney. [Calyptocephalella caudiverbera]
Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:7189228
Distinct angiotensin II receptor in primary cultures of glial cells from rat brain
Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5315449

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
ANGIOTENSIN
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BODY
CALCIUM COMPOUNDS
CARDIOVASCULAR AGENTS
CATIONS
CELL CULTURES
CHARGED PARTICLES
CHEMICAL REACTIONS
CHEMISTRY
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GLOBULINS
GROWTH FACTORS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IONS
ISOTOPES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SKIN
TISSUES
VASOCONSTRICTORS