Flow cytometry apparatus
This paper describes an apparatus for orienting cells in a sheath fluid in a otometer/sorter. It comprises: flow chamber; means for flowing the sheath fluid through the flow chamber along a direction of flow; means for obstructing the flow of the sheath fluid in the flow chamber with a first dimension, which extends substantially across the flow chamber and is substantially perpendicular to the direction of flow and with a thickness perpendicular to the first dimension of the obstructing means wherein the sheath fluid flows around the thickness so that the sheath fluid converges in only one dimension at the downstream edge of the means for obstructing; and means for introducing the cells through the means for obstructing the flow to the region where the sheath fluid converges in only one dimension in the sheath fluid to orient the cells, with an aperture wherein as the cells pass from the means for introducing the cells to the region where the sheath fluid converges the cells pass through the aperture with a cross-sectional length substantially less than or equal to the thickness of the means for obstructing the flow.
- Assignee:
- Dept. of Energy, Washington, DC (USA)
- Patent Number(s):
- US 4988619; A
- Application Number:
- PPN: US 7-126153
- OSTI ID:
- 5059246
- Resource Relation:
- Patent File Date: 30 Nov 1987
- Country of Publication:
- United States
- Language:
- English
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