Engineering multiple properties of a protein by combinatorial mutagenesis
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
- Univ. of Chicago, IL (United States)
- Los Alamos National Laboratory, NM (United States)
A method for simultaneously engineering multiple properties of a protein, based on the observed additivity of effects of individual mutations, is presented. The authors show that, for the gene V protein of bacteriophage f1, effects of double mutations on both protein stability and DNA binding affinity are approximately equal to the sums of the effects of the constituent single mutations. This additivity of effects implies that it is possible to deliberately construct mutant proteins optimized for multiple properties by combination of appropriate single mutations chosen from a characterized library. 45 refs., 3 figs., 1 tab.
- OSTI ID:
- 5053868
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 90:18; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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