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Incorporation of metabolites into glycogen and lipids of the oyster, crassostrea virginica

Conference · · Federation Proceedings, Federation of American Societies for Experimental Biology; (USA)
OSTI ID:5051772
;  [1]
  1. Howard Univ., Washington, DC (USA)

Groups of oysters, either fed or unfed, were exposed to U-{sup 14}C labelled D-glucose, L-asp, L-leu, L-ala or acetate for 6 hrs. Except for the glucose trials, the disappearance of radioactivity from the saline of the unfed oysters was greater (83%) than for the fed animals (65%). With glucose, 88% of the radioactivity disappeared in each trial. The specific radioactivity of glycogen isolated from oysters exposed to labelled glucose, asp and ala was 1283, 468 and 8.22 dpm/mg glycogen respectively. Radioactivity was found primarily in the triacylglycerols and phospholipids (PL) in fed oysters and in PL only in unfed oysters. Phosphatidyl choline, phosphatidyl ethanolamine, and a fraction containing phosphatidyl serine and phosphatidyl inositol, had 32%, 25% and 35-40% of the radioactivity respectively. Incorporation of total radioactivity into PL was 70% lower in unfed vs. fed trials, but the distribution of counts among the phospholipids classes was unchanged. Glycogenesis does not appear to be a significant pathway in the oyster. Apparently well-fed oysters are able to store excess dietary calories as lipid. During periods of starvation exogenous small metabolites along with glucose from glycogen are catabolized.

OSTI ID:
5051772
Report Number(s):
CONF-870644--
Journal Information:
Federation Proceedings, Federation of American Societies for Experimental Biology; (USA), Journal Name: Federation Proceedings, Federation of American Societies for Experimental Biology; (USA) Vol. 46:6; ISSN 0014-9446; ISSN FEPRA
Country of Publication:
United States
Language:
English