Tumor necrosis factor. alpha. induces proteins that bind specifically to. kappa. B-like enhancer elements and regulate interleukin 2 receptor. alpha. -chain gene expression in primary human T lymphocytes

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.7.2331· OSTI ID:5047234

Lowenthal, J W; Ballard, D W; Boehnlein, E; Greene, W C ^[1]

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1. Duke Univ. Medical Center, Durham, NC (USA)

The authors have investigated the biochemical basis for the activation of interleukin 2 receptor {alpha}-subunit (IL-2R{alpha}) gene expression in primary human T lymphocytes by a cytokine (tumor necrosis factor {alpha}), a T-cell mitogen (phorbol 12-myristate 13-acetate), and the transactivator protein (Tax) from the type I human T-cell leukemia virus. Using in vivo transfection techniques specifically designed for these primary T cells in conjunction with in vitro gel retardation and DNA footprinting assays, the authors found that activation of the IL-2R{alpha} promoter by each of these agents involves the induction of nuclear proteins that specifically interact with a {kappa}B-like enhancer element. DNA-protein crosslinking studies revealed that primary T cells express at least three different inducible DNA-binding proteins that specifically interact with this IL-2R{alpha} {kappa}B element.

• OSTI ID: 5047234
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:7; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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BIOLOGICAL MATERIALS
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BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
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MITOGENS
NECROSIS
NUCLEIC ACIDS
OLIGONUCLEOTIDES
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
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PLASMIDS
POLYMERIZATION
PRIMATES
PROTEINS
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