Immunoreactive atrial natriuretic peptide in the guinea pig spleen

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90132-X· OSTI ID:5045547

Vollmar, A M; Friedrich, A; Schulz, R ^[1]

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1. Univ. of Munich (Germany, F.R.)

The presence of immunoreative ANP precursor-like material in the guinea pig spleen is suggested. This is based on the following experimental evidence: An acidic extract of guinea pig spleen analyzed by Sephadex G-50 gel filtration contained 4.6 pmol/g wet tissue immunoreactive atrial natriuretic peptide (IR-ANP), IR-ANP coeluting with 15 kDa synthetic ANP (2-126). Gel filtrated IR-ANP material was further submitted to reverse phase high performance liquid chromatography and monitored by radioimmunoassay employing two antisera. One antiserum recognizes the C-terminal of ANP (1-126), the second is directed against the N-terminal sequence. Both antisera revealed material eluting with synthetic ANP (2-126). Furthermore, immunohistochemical analysis suggests this ANP-like material to be localized mainly at the periphery of the white pulp of the spleen. These findings link ANP with the immune system.

• OSTI ID: 5045547
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 45:14; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BIOASSAY
BIOLOGICAL LOCALIZATION
BODY
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
GUINEA PIGS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RETICULOENDOTHELIAL SYSTEM
RODENTS
SEPARATION PROCESSES
SPLEEN
TISSUES
TRACER TECHNIQUES
VERTEBRATES