Use of membrane vesicles as a simplified system for studying transport of auxin. Progress report

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:5044442

Goldsmith, M H

The accumulation of indoleacetic acid (IAA) inside microsomal vesicles depends on the presence of a pH gradient and is reversible when the ..delta..pH is collapsed by ionosphores. Accumulation is stimulated by either napthylphthalamic acid or TIBA. The accumulation of IAA by the vesicles can be saturated. At concentrations of 1 ..mu..M or less, IAA, synthetic auxins, or auxin antagonists do not affect the pH gradient, but decrease the accumulation of /sup 3/H-IAA, and therefore compete specifically for uptake. Concentrations of 10 ..mu..M and above, uptake of either the auxins or weak acids is sufficient to overcome the buffering capacity of the solution within the vesicles. The collapse of the pH gradient by such high concentrations affects uptake of either /sup 3/H-IAA or /sup 14/C-BA to similar extents and thus is nonspecific. 3 refs.

• Research Organization: Yale Univ., New Haven, CT (USA)
• DOE Contract Number: FG02-85ER13337
• OSTI ID: 5044442
• Report Number(s): DOE/ER/13337-1; ON: DE86002264
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
AUXINS
AZAARENES
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRIFUGATION
HERBICIDES
HETEROCYCLIC COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPOSOMES
MEMBRANE TRANSPORT
MEMBRANES
MICROSOMES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PESTICIDES
PH VALUE
PLANT GROWTH REGULATORS
PLANTS
PYRROLES
REACTION KINETICS
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION