Influence of calcium ion on ethanol tolerance of Saccharomyces bayanus and alcoholic fermentation by yeasts

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Applied and Environmental Microbiology; (USA)

OSTI ID:5043003

Nabais, R C; Sa-Correia, I; Viegas, C A; Novais, J M ^[1]

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1. Instituto Superior Tecnico, Lisbon (Portugal)

The addition of Ca{sup 2+} (as CaCl{sub 2}) in optimal concentrations (0.75 to 2.0 mM) to a fermentation medium with a trace contaminating concentration of Ca{sup 2+} (0.025 mM) led to the rapid production of higher concentrations of ethanol by Saccharomyces cerevisiae, Saccharomyces bayanus, and Kluyveromyces marxianus. The positive effect of calcium supplementation (0.75 mM) on alcoholic fermentation by S. bayanus was explained by the increase in its ethanol tolerance. For lethal concentrations of ethanol, the specific death rates were minimal for cells that were grown and incubated with ethanol in medium with an optimal concentration of Ca{sup 2+}, maximal for cells grown and incubated with ethanol in unsupplemented medium, and intermediate for cells grown in unsupplemented medium and incubated with ethanol in calcium-supplemented medium. The effect of Ca{sup 2+} on the acidification curve of energized cells in the presence of ethanol was found to be closely associated with its protective effect on growth, fermentation, and viability.

• OSTI ID: 5043003
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 54:10; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALKALINE EARTH METAL COMPOUNDS
BIOCONVERSION
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CATIONS
CHARGED PARTICLES
ETHANOL
EUMYCOTA
FERMENTATION
FUNGI
GROWTH
HYDROXY COMPOUNDS
IONS
MICROORGANISMS
MORTALITY
ORGANIC COMPOUNDS
PLANTS
SACCHAROMYCES
SENSITIVITY
TOLERANCE
TOXICITY
YEASTS