Proteins of human urine. III. Identification and two-dimensional electrophoretic map positions of some major urinary proteins

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5032360

Edwards, J J; Tollaksen, S L; Anderson, N G

The proteins of human urine have been mapped by high-resolution two-dimensional electrophoresis, utilizing the ISO-DALT system. Wide-range pH gradients and narrow-range acid gradients were both used in the first-dimension separations. The patterns revealed proteins ranging in relative molecular mass from 10 000 to 90 000. Proteins identified in the map included transferrin, albumin, hemopexin, ..cap alpha../sub 2/-HS glycoprotein, ..cap alpha../sub 1/-antitrypsin, Gc globulin, ..cap alpha../sub 1/-acid glycoprotein, Zn ..cap alpha../sub 2/-glycoprotein, retinol binding protein, ..beta../sub 2/-microglobulin, the immunoglobulin light chains, and MAUP (most acid urinary protein). The use and utility of internal-charge and molecular-mass standards are described. We used electrophoretic transfer of proteins to nitrocellulose sheets and subsequent detection by immunological methods to identify some proteins.

• Research Organization: Argonne National Lab., IL
• DOE Contract Number: W-31-109-ENG-38
• OSTI ID: 5032360
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ELECTROPHORESIS
DIAGNOSTIC USES
PROTEINS
URINE
ALBUMINS
GLUCOPROTEINS
IMMUNOLOGY
MAN
MOLECULAR WEIGHT
NITROCELLULOSE
PH VALUE
TRANSFERRIN
ANIMALS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CARBOHYDRATES
CELLULOSE ESTERS
CHEMICAL EXPLOSIVES
ESTERS
EXPLOSIVES
GLOBULINS
GLOBULINS-BETA
MAMMALS
MATERIALS
METALLOPROTEINS
NITRIC ACID ESTERS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PRIMATES
SACCHARIDES
USES
VERTEBRATES
WASTES
550200* - Biochemistry