Structural requirements for galanin interaction with receptors from pancreatic beta cells and from brain tissue of the rat

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Peptides (Fayetteville, New York); (USA)

DOI:https://doi.org/10.1016/0196-9781(89)90109-5· OSTI ID:5031544

Lagny-Pourmir, I; Lorinet, A M; Yanaihara, N; Laburthe, M ^[1]

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1. Unite de Recherches sur la Differenciation et la Neuroendocrinologie de Cellules Digestives, Batiment INSERM, Villejuif (France)

The binding activity of several galanin fragments and analogs was measured on specific receptors present in rat brain and the rat pancreatic beta cell line Rin m 5F. In both tissues it was observed that: (1) galanin(3-29), galanin(10-29) and (Ile2)-galanin were ineffective for inhibiting ({sup 125}I) galanin binding and (2) active peptides had the following rank order of potency: galanin(1-29) greater than (Ac-Trp2)-galanin(2-29) greater than galanin(2-29) greater than galanin(1-15) greater than (Phe2)-galanin greater than (Tyr2)-galanin. It was concluded that the N-terminal portion of galanin is very important for interaction with central or peripheral receptors. The aromatic amino acid in position 2 (Trp in native galanin) plays a crucial role.

• OSTI ID: 5031544
• Journal Information: Peptides (Fayetteville, New York); (USA), Vol. 10:4; ISSN 0196-9781
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRAIN
BIOCHEMISTRY
PEPTIDES
BIOCHEMICAL REACTION KINETICS
AMINO ACIDS
CELL CULTURES
CELL MEMBRANES
IN VITRO
IODINE 125
NERVES
PANCREAS
RATS
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
GLANDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques