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Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens

Journal Article · · Journal of Bacteriology; (USA)
OSTI ID:5031450
;  [1]
  1. Hebrew Univ. of Jerusalem (Israel)
+ Show Author Affiliations

The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of {sup 32}P-labeled cells grown in various media, and an enzyme responsible for its formation from GTP was found to be present in cell-free preparations. Cellulose synthesis in vivo was quantitatively assessed with ({sup 14}C)glucose as a tracer. The organism produced cellulose during growth in the absence of plant cells, and this capacity was retained in resting cells. Synthesis of a cellulosic product from UDP-glucose in vitro with membrane preparations was markedly stimulated by c-di-GMP and its precursor GTP and was further enhanced by Ca2+. The calcium effect was attributed to inhibition of a c-di-GMP-degrading enzyme shown to be present in the cellulose synthase-containing membranes.

OSTI ID:
5031450
Journal Information:
Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:12; ISSN JOBAA; ISSN 0021-9193
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AROMATICS
AZAARENES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CELLULOSE
DAYS LIVING RADIOISOTOPES
ENZYMES
GLUCOSE
GROWTH
GUANOSINE
HETEROCYCLIC COMPOUNDS
HEXOSES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MEMBRANES
MICROORGANISMS
MONOSACCHARIDES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
POLYSACCHARIDES
PURINES
RADIOISOTOPES
RIBOSIDES
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
TRANSFERASES