Interleukin 1. alpha. inhibits prostaglandin E sub 2 release to suppress pulsatile release of luteinizing hormone but not follicle-stimulating hormone

Journal Article · Mon Apr 01 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.7.2763· OSTI ID:5026602

Rettori, V; McCann, S M ^[1]; Gimeno, M F ^[2]; Karara, A ^[3]; Gonzalez, M C ^[4]

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1. Univ. of Texas Southwestern Medical Center, Dallas (United States)
2. CEFYBO, Buenos Aires (Argentina)
3. Vanderbilt Univ., Nashville, TN (United States)
4. Univ. de La Laguno, Tenerife (Spain)

Interleukin 1{alpha} (IL-1{alpha}), a powerful endogenous pyrogen released from monocytes and macrophages by bacterial endotoxin, stimulates corticotropin, prolactin, and somatotropin release and inhibits thyrotropin release by hypothalamic action. The authors injected recombinant human IL-1{alpha} into the third cerebral ventricle, to study its effect on the pulsatile release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in conscious, freely moving, ovariectomized rats. Intraventricular injection of 0.25 pmol of IL-1{alpha} caused an almost immediate reduction of plasma LH concentration. To determine the mechanism of the suppression of LH release, mediobasal hypothalamic fragments were incubated in vitro with IL-1{alpha} (10 pM) and the release of LH-releasing hormone (LHRH) and prostaglandin E{sub 2} into the medium was measured by RIA in the presence or absence of nonrepinephrine. 1{alpha} reduced basal LHRH release and blocked LHRH release induced by nonrepinephrine. In conclusion, IL-1{alpha} suppresses LH but not FSH release by an almost complete cessation of pulsatile release of LH in the castrated rat. The mechanism of this effect appears to be by inhibition of prostaglandin E{sub 2}-mediated release of LHRH.

• OSTI ID: 5026602
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:7; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOASSAY
BIOLOGICAL EFFECTS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CASTRATION
DIAGNOSTIC TECHNIQUES
DRUGS
FSH
GONADOTROPINS
GROWTH FACTORS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LH
LYMPHOKINES
MAMMALS
MEDICINE
MITOGENS
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RATS
RODENTS
SURGERY
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES