Characterization of insulin-like growth factor I and epidermal growth factor receptors in meningioma

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Neurosurgery; (USA)

DOI:https://doi.org/10.3171/jns.1989.71.4.0538· OSTI ID:5026565

Kurihara, M; Tokunaga, Y; Tsutsumi, K; Kawaguchi, T; Shigematsu, K; Niwa, M; Mori, K ^[1]

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1. Nagasaki Univ. School of Medicine (Japan)

Receptors for insulin-like growth factor I (IGF-I) and epidermal growth factor (EGF) were localized and characterized in eight samples of human meningioma (four fibrous, two meningothelial, and two angioblastic types), using quantitative autoradiographic techniques. Effects of both growth factors on deoxyribonucleic acid (DNA) synthesis in the cultured meningioma cells were examined. High numbers of specific binding sites for both IGF-I and EGF were homogeneously present in tissue sections derived from fibrous and meningothelial types of meningiomas, whereas binding sites for these growth factors were not detectable in adjacent leptomeninges. While relatively large numbers of IGF-I binding sites were located in the wall of the intratumoral vasculature, the number of binding sites in the stromal component was lower in angioblastic-type meningiomas, including a low number of EGF binding sites detected only in the stromal portion. Scatchard analysis revealed the presence of a single class of high-affinity binding sites for both IGF-I and EGF in the meningiomas examined (dissociation constant (Kd) = 0.6 to 2.9 nM, and the maximum number of binding sites (Bmax) = 16 to 80 fmol/mg for IGF-I; and Kd = 0.6 to 4.0 nM, Bmax = 3 to 39 fmol/mg for EGF). Both growth factors increased the synthesis of DNA, in a dose-dependent manner, as measured by 3H-thymidine incorporation. The combination of IGF-I and EGF synergistically stimulated the synthesis of DNA, and the effects seen with 10% fetal bovine serum could be reproduced at a concentration of 10(-10) M. These observations can be interpreted to mean that both IGF-I and EGF may be involved in the growth modulation of meningiomas, possibly through paracrine or autocrine mechanisms.

• OSTI ID: 5026565
• Journal Information: Journal of Neurosurgery; (USA), Journal Name: Journal of Neurosurgery; (USA) Vol. 71:4; ISSN 0022-3085; ISSN JONSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL LOCALIZATION
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MENINGES
MITOGENS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
RECEPTORS
RIBOSIDES
THYMIDINE
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES