sup 13 C NMR for the assessment of human brain glucose metabolism in vivo

Journal Article · Tue Jul 02 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00240a002· OSTI ID:5025763

Beckman, N; Seelig, J ^[1]; Turkalj, I; Keller, U ^[2]

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1. Univ. of Basel (Switzerland)
2. Univ. Hospital, Basel (Switzerland)

Proton-decoupled {sup 13}C NMR spectra of the human head were obtained during hyperglycemic glucose clamping using intravenous infusions of (1-{sup 13}C)glucose in normal volunteers. In addition to {sup 13}C signals of mobile lipids, a variety of new metabolite resonances could be resolved for the first time in the human brain. At an enrichment level of 20% (1-{sup 13}C)glucose, the signals of {alpha}- and {beta}-glucose at 92.7 and 96.6 ppm, respectively, could be detected in the human brain after only an infusion period of 15 minutes. The spatial localization of the different regions of interest was confirmed by {sup 13}C NMR spectroscopic imaging with a time resolution of 9 minutes. Increasing the enrichment level to 99% (1-{sup 13}C)glucose not only improved the time resolution but allowed the detection of metabolic breakdown products of (1-{sup 13}C)glucose. The time course of {sup 13}C label incorporation into the C{sub 2}, C{sub 3}, and C{sub 4} resonances of glutamate/glutamine and into lactate could be recorded in the human brain. These results suggest the possibility of obtaining time-resolved, spatially selective, and chemically specific information on the human body.

• OSTI ID: 5025763
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:26; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINO ACIDS
ANIMALS
BODY
BRAIN
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
EVEN-ODD NUCLEI
GLUCOSE
GLUTAMIC ACID
HEXOSES
IN VIVO
INFUSION
INJECTION
INTAKE
INTRAVENOUS INJECTION
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
MAMMALS
MAN
METABOLISM
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
RESONANCE
SACCHARIDES
STABLE ISOTOPES
VERTEBRATES