Modeling hexavalent chromium reduction in Escherichia coli 33456
- Univ. of Kentucky, Lexington, KY (United States). Dept. of Civil Engineering
A model based on the analysis of the mechanism of enzymatic reaction was developed to characterize the rate and extent of microbial reduction of hexavalent chromium in Escherichia coli 33456. A finite reduction capacity (R[sub c]) was proposed and incorporated into the enzymatic model to regulate the toxicity effect on cells due to the oxidizing power of Cr(6). The parameter values were determined by nonlinear least-square analysis using experimental data of anaerobic cultures. The obtained parameters were then used to predict (Cr(6)) reduction in aerobic cultures along with a modification term of uncompetitive inhibition from molecular oxygen. The applicability of the developed model was demonstrated through excellent prediction of the results of batch studies conducted over range of initial Cr(6) concentrations, initial cell densities, and DO levels. A sensitivity analysis revealed that the parameters obtained using the experimental data were unique, and neither change in K[sub c], the half-velocity constant, at high initial (Cr(6)) concentrations nor change in R[sub c], the reduction capacity, at low initial (Cr(6)) concentrations was sensitive to model prediction.
- OSTI ID:
- 5024452
- Journal Information:
- Biotechnology and Bioengineering; (United States), Vol. 43:4; ISSN 0006-3592
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMIUM
METABOLISM
TOXICITY
ESCHERICHIA COLI
GROWTH
OXIDOREDUCTASES
ENZYME ACTIVITY
MATHEMATICAL MODELS
BACTERIA
ELEMENTS
ENZYMES
METALS
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
TRANSITION ELEMENTS
560300* - Chemicals Metabolism & Toxicology