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Phosphatidylinositol(4,5)bisphosphate and phosphatidylinositol(4)phosphate in plant tissues. [Pisum sativum]

Journal Article · · Plant Physiology; (USA)
DOI:https://doi.org/10.1104/pp.89.3.888· OSTI ID:5023442
; ;  [1];  [2];  [3];  [4]
  1. Institute of Animal Physiology and Genetics Research, Cambridge (England)
  2. Univ. of East Anglia, Norwich (England)
  3. Univ. of Amsterdam (Netherlands)
  4. John Innes Institute, Norwich (England)

Pea (Pisum sativum) leaf discs or swimming suspensions of Chlamydomonas eugametos were radiolabeled with ({sup 3}H)myo-inositol or ({sup 32}P)Pi and the lipids were extracted, deacylated, and their glycerol moieties removed. The resulting inositol trisphosphate and bisphosphate fractions were examined by periodate degradation, reduction and dephosphorylation, or by incubation with human red cell membranes. Their likely structures were identified as D-myo-inositol(1,4,5)trisphosphate and D-myo-inositol(1,4,)-bisphosphate. It is concluded that plants contain phosphatidylinositol(4)phosphate and phosphatidylinositol(4,5)bisphosphate; no other polyphosphoinositides were detected.

OSTI ID:
5023442
Journal Information:
Plant Physiology; (USA), Journal Name: Plant Physiology; (USA) Vol. 89:3; ISSN 0032-0889; ISSN PLPHA
Country of Publication:
United States
Language:
English