Early and late modes of DNA replication in ultraviolet irradated Syrian hamster embryo cells
Journal Article
·
· Biophys. J.; (United States)
The nature of DNA replication in UV irradiated Syrian hamster embryo cells (HEC) was investigated by measuring the size distribution of nascent daughter strand DNA. During the early mode, nascent strands are made in smaller pieces than in nonirradiated cells. The late mode begins when nascent strands recover to normal size. This was observed in HEC 5 h post-UV. When the late mode is operational, nascent strands elongate to parental size in <2 h, whereas >3 h are required during early mode function. Evidence from split dose experiments demonstrates that the recovery of the size of nascent strands is not due to enhanced gap filling. Furthermore, pyrimidine dimers are probably recognized differently by the replication complex during early and late mode DNA synthesis. The late mode of replication could account for the ability of HEC to survive UV irradiation even though they are inefficient in both excision and postreplication repair.
- Research Organization:
- National Cancer Inst., Bethesda, MD
- OSTI ID:
- 5023375
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 31:2; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CELL CULTURES
CELL DIVISION
DIMERS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
FRACTIONATED IRRADIATION
GENETICS
HAMSTERS
HETEROCYCLIC COMPOUNDS
IRRADIATION
MAMMALS
MITOSIS
MORPHOLOGICAL CHANGES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
ULTRASTRUCTURAL CHANGES
ULTRAVIOLET RADIATION
VERTEBRATES
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGY
CELL CULTURES
CELL DIVISION
DIMERS
DNA
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
FRACTIONATED IRRADIATION
GENETICS
HAMSTERS
HETEROCYCLIC COMPOUNDS
IRRADIATION
MAMMALS
MITOSIS
MORPHOLOGICAL CHANGES
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
ULTRASTRUCTURAL CHANGES
ULTRAVIOLET RADIATION
VERTEBRATES