Characterization of the spontaneous DNA-synthesizing and/or IgG-secreting cells in peripheral blood from patients with systemic lupus erythematosus
Characterization of the cells responsible for spontaneous DNA synthesis and/or IgG secretion in systemic lupus erythematosus (SLE) was undertaken by fractionation of the peripheral blood mononuclear cells (PBM). Each fraction was analyzed for its capacity to incorporate (/sup 3/H)thymidine ((/sup 3/H)TdR) and secrete IgG without mitogen. The non-E rosette-forming cell (non-ERRC) fraction, which consisted of the surface immunoglobulin-positive (sIg(+)) cells and null cells, revealed a markedly increased spontaneous DNA synthesis (620.0 +/- 586.9 cpm) during the first hour of culture and an elevated spontaneous IgG secretion (8639 +/- 2630 ng/ml) during 9 days of culture. Of particular interest was the finding that both increased responses were conducted by the null cells; the null cell population had approximately a fourfold relative increase of (/sup 3/H)TdR incorporation and a 60-fold relative increase of IgG secretion compared with the sIg(+) cell population. These results suggest that SLE patients have a small population of preactivated B-cell lineage cells, which lack sIg or have a very low density of sIg.
- OSTI ID:
- 5021099
- Journal Information:
- Clin. Immunol. Immunopathol.; (United States), Journal Name: Clin. Immunol. Immunopathol.; (United States) Vol. 1; ISSN CLIIA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODY FORMATION
AZINES
BACTERIAL DISEASES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISEASES
DNA
GLOBULINS
HETEROCYCLIC COMPOUNDS
IMMUNOGLOBULINS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LUPUS
LYMPHOCYTES
MATERIALS
MONOCYTES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATIENTS
PROTEINS
PYRIMIDINES
RIBOSIDES
SKIN DISEASES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUBERCULOSIS