Determination of the intracellular concentration of ethanol in Saccharomyces cerevisiae during fermentation

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5020832

Dombek, K M; Ingram, L O

Considerable controversy exists concerning the intracellular concentration of ethanol in Saccharomyces cerevisiae during fermentation. This controversy results from problems in the measurement of the intracellular concentration of compounds like ethanol, which are being produced rapidly by metabolism and potentially diffuse rapidly from the cell. A new method was used for the determination of intracellular ethanol based on the exclusion of (/sup 14/C)sorbitol to estimate the aqueous cell volume. This method avoided many of the technical problems in previous reports. Results indicate that the intracellular concentrations of ethanol in fermenting suspension of S. cerevisiae are less than or equal to those in the extracellular environment and do not increase to the high levels previously reported even during the most active stages of batch fermentation.

• Research Organization: Univ. of Florida, Gainesville
• OSTI ID: 5020832
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 51:1; ISSN AEMID
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
ALDEHYDES
BIOCONVERSION
BIOLOGICAL ACCUMULATION
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
ETHANOL
FERMENTATION
FUNGI
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
TRACER TECHNIQUES
YEASTS