Construction of time-resolved fluorescence detector for amino compounds after high-performance liquid chromatography using europium chelate

Journal Article · Thu May 15 04:00:00 EDT 1997 · Analytical Chemistry (Washington)

DOI:https://doi.org/10.1021/ac961224w· OSTI ID:501951

Iwata, Tetsuo; Senda, Masaaki; Kurosu, Yasuyuki ^[1]; Tsuji, Akio; Maeda, Masako ^[2]

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1. JASCO Technical Research Laboratories Corporation, Tokyo (Japan)
2. Showa Univ., Tokyo (Japan)

We have constructed a simple, low-cost, time-resolved fluorescence detector (TRFD) for analysis of amino compounds with high-performance liquid chromatography using europium (Eu{sup 3+}) chelates, which consists of a dc-operated xenon lamp, two synchronized mechanical choppers, and spectroscopic filters. Amino compounds derivatized by isothiocyanobenzyl EDTA with Eu{sup 3+} chelate are mixed with an enhancer solution postcolumn and detected by the long TRFD. Taking advantage of the long fluorescence lifetime of the Eu{sup 3+} chelates, high selectivity against background fluorescein is achieved. To demonstrate the usefulness of the TRFD, fundamental performance test comparison with the conventional fluorescence detector was carried our. Details of the system are also described. 12 refs., 5 figs., 1 tab.

• OSTI ID: 501951
• Journal Information: Analytical Chemistry (Washington), Journal Name: Analytical Chemistry (Washington) Journal Issue: 10 Vol. 69; ISSN ANCHAM; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DESIGN
EUROPIUM COMPLEXES
FLUORESCENCE
LIQUID COLUMN CHROMATOGRAPHY
MEASURING INSTRUMENTS
ORGANIC COMPOUNDS