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Determination of bioactivity of chemical fractions of liquid wastes using freshwater and saltwater algae and crustaceans

Journal Article · · Environ. Sci. Technol.; (United States)
DOI:https://doi.org/10.1021/es00109a012· OSTI ID:5019038
 [1];
  1. US Environmental Protection Agency, Gulf Breeze, FL

Complex wastes from industrial and municipal outfalls were fractionated chemically and tested for toxicity with freshwater and saltwater algae and crustaceans. The organic fraction of each waste was subfractionated into acid-, base-, and neutral-extractable portions, and the inorganic fraction was subfractionated into its anion and cation components. All wastes affected growth of the algae Skeletonema costatum (saltwater) and Monoraphidium capricornutum (freshwater) or survival of Mysidopsis bahia (saltwater) and Daphnia magna (freshwater). Usually, bioactivity was limited to one or two subfractions. In some cases, algal growth was stimulated by a fraction or subfraction, whereas stimulation was not detected in whole waste. It is suggested that fractionation must be done in order to estimate the full potential impact of complex wastes on aquatic systems. The method can also be used to identify toxic factors before application of cost-effective control technology.

OSTI ID:
5019038
Journal Information:
Environ. Sci. Technol.; (United States), Journal Name: Environ. Sci. Technol.; (United States) Vol. 17:3; ISSN ESTHA
Country of Publication:
United States
Language:
English