Microclones derived from the mouse chromosome 7 D-E bands map within the proximal region of the c14CoS deletion in albino mutant mice

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Genomics; (United States)

DOI:https://doi.org/10.1016/0888-7543(91)90453-L· OSTI ID:5018197

Toenjes, R R.W.; Weith, A; Rinchik, E M; Winking, H; Carnwath, J W; Kaliner, B; Paul, D ^[1]

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1. Department of Cell Biology, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover (West Germany)

A group of radiation-induced perinatal-lethal deletions that include the albino (c) locus on mouse chromosome 7 causes failure of expression of various hepatocyte-specific genes when homozygous. The transcription of such genes could be controlled in trans by a regulatory gene(s) located within the proximal region of the C14CoS deletion. To identify this potential regulatory gene, a microclone library was established from microdissected D and E bands of chromosome 7. Three nonoverlapping microclones (E305, E336B, and E453B) hybridizing with wildtype but not with C14CoS/C14CoS DNA were isolated. E336B represents a single-copy DNA fragment, whereas E305 and E453B hybridized with 3 and 10 EcoRI DNA restriction fragments, respectively. All fragments map exclusively within the deletion. The microclones hybridized to DNA of viable C6H/C14CoS deletion heterozygotes but not to DNA of homozygotes for the lethal mutation c10R75M, which belongs to the same complementation group as c14CoS. DNA of viable homozygous mutant C62DSD, which carries a deletion breakpoint proximal to that of c6H, hybridized only with E453B. This microclone identified 6 EcoRI restriction fragments in C62DSD/C62DSD DNA. The results demonstrate that of the isolated microclones, E453B identifies a locus (D7RT453B) that maps closest to the hsdr-1 (hepatocyte-specific developmental regulation) locus, which maps between the proximal breakpoints of deletions c10R75M and c62DSD.

• OSTI ID: 5018197
• Journal Information: Genomics; (United States), Vol. 10:3; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CLONE CELLS
GENETIC MAPPING
GENE MUTATIONS
RADIOINDUCTION
LIVER CELLS
GENE REGULATION
CHROMOSOMES
DNA HYBRIDIZATION
GENES
GLUCOCORTICOIDS
LETHAL MUTATIONS
MICE
MUTANTS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
CELL CULTURES
CORTICOSTEROIDS
HORMONES
HYBRIDIZATION
HYDROXY COMPOUNDS
KETONES
MAMMALS
MAPPING
MUTATIONS
ORGANIC COMPOUNDS
PREGNANES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals