DNA sequencing process using stable isotopes
Patent
·
OSTI ID:5018156
This patent describes a DNA sequencing process using individual stable isotopes that are associated with individual deoxynucleotides and/or terminators. It comprises introducing template DNA strands with primer hybridization into a solution containing deoxynucleoside triphosphates (dNTP) having thymine, adenine, cytosine, and guanine bases; DNA polymerase; buffer; magnesium ion, and dideoxynucleoside triphosphate (ddNTP) wherein the ratio of dNTP to ddNTP is selected, the ddNTPs causing replication termination when incorporated into the replicated strand as dideoxynucleotide at a frequency dependent upon the ratio of dNTP to ddNTP; incorporating a specific stable isotope into at least one of the dNTPs and or ddNTPs such that a terminated strand has a specific stable isotope associated with each terminator at its end; separating the terminated strands by performing gel electrophoresis thereof; and determining where each the specific stable isotope is located on the gel.
- Assignee:
- Atom Sciences, Inc., Oak Ridge, TN (USA)
- Patent Number(s):
- A; US 5002868
- Application Number:
- PPN: US 7-221734
- OSTI ID:
- 5018156
- Country of Publication:
- United States
- Language:
- English
Similar Records
Method of altering double-stranded DNA
Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I
Genomic footprinting in mammalian cells with ultraviolet light
Patent
·
Tue May 05 00:00:00 EDT 1987
·
OSTI ID:5881291
Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I
Journal Article
·
Thu Jun 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5429166
Genomic footprinting in mammalian cells with ultraviolet light
Journal Article
·
Sat Jul 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5406994