Characterization of antibody-chelator conjugates: Determination of chelator content by terbium fluorescence titration

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Bioconjugate Chemistry; (United States)

DOI:https://doi.org/10.1021/bc00007a011· OSTI ID:5018059

Brandt, K D; Schnobrich, K E; Johnson, D K ^[1]

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1. Abbott Laboratories, Department 90M, Abbott Park, IL (United States)

Fluorescence titrations were performed by adding varying mole ratios of terbium(III) to antibody conjugates formed by benzyl isothiocyanate derivatives of three different polyaminopolycarboxylate chelators (NTA, EDTA, and DTPA) and the results compared to values for average chelator content obtained by cobalt-57 binding assays. For two different murine monoclonal antibodies, the average chelator content obtained by terbium fluorescence titration correlated closely with that measured by the cobalt-57 binding assay. It is concluded that lanthanide fluorescence titrations provide a useful alternative to radiometal binding assays for the determination of chelator content in protein-chelator conjugates.

• OSTI ID: 5018059
• Journal Information: Bioconjugate Chemistry; (United States), Journal Name: Bioconjugate Chemistry; (United States) Vol. 2:1; ISSN 1043-1802; ISSN BCCHE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
CARCINOEMBRYONIC ANTIGEN
CHELATING AGENTS
CHEMICAL COMPOSITION
COBALT 57
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
FLUORESCENCE
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
MAMMALS
METALS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
RADIOASSAY
RADIOISOTOPES
RARE EARTHS
RODENTS
TERBIUM
TRACER TECHNIQUES
VERTEBRATES