Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Quantitative requirement for ATP for active transport in isolated renal cells

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5017456
; ;
We investigated the quantitative relationship between cellular ATP concentration and Na+-K+-ATPase activity as measured by ouabain-sensitive 86Rb influx in rabbit proximal renal cells. Cellular ATP was reduced in a stepwise manner by rotenone (10(-7) to 10(-5) M) and was increased by 10 mM adenosine. During these maneuvers, ouabain-sensitive 86Rb influx was linearly related to cellular ATP and did not saturate up to 9.9 mM ATP. In contrast, Na+-K+-ATPase activity in membranes prepared from these cells saturated at 2.0 mM ATP at various sodium (10-100 mM) and potassium (4-100 mM) concentrations. Sodium-dependent phosphate uptake and alpha-methylglucoside (alpha-MG) uptake were both inhibited to a similar degree when cellular ATP was reduced. We conclude that 1) the ATP requirement for saturation of Na+-K+-ATPase is higher in intact renal cells than in the membranes, and 2) the uptake of phosphate and alpha-MG are similarly influenced by reduction in ATP. This effect of ATP on phosphate and AMG uptake is most likely an indirect one and is secondary to changes in the sodium gradient across the cell.
OSTI ID:
5017456
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
Country of Publication:
United States
Language:
English

Similar Records

Characterization of bicarbonate-dependent potassium uptake in cultured corneal endothelial cells
Journal Article · Fri Mar 31 23:00:00 EST 1989 · Invest. Ophthalmol. Visual Sci.; (United States) · OSTI ID:6155915
Mechanisms of mercurial and arsenical inhibition of tyrosine absorption in intestine of the winter flounder Pseudopleuronectus americanus
Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Toxicology and Applied Pharmacology; (USA) · OSTI ID:6867630
Membrane potentials and sugar transport by ATP-depleted intestinal cells: effect of anion gradients
Journal Article · Sun Dec 31 23:00:00 EST 1978 · Am. J. Physiol.; (United States) · OSTI ID:6794376

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ALDEHYDES
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMAL CELLS
ANIMALS
ATP
ATP-ASE
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ELEMENTS
ENZYMES
FUNCTIONS
GLUCOSE
HEXOSES
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHOHYDROLASES
PHOSPHORUS COMPOUNDS
RABBITS
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOPES
SACCHARIDES
SODIUM
SOMATIC CELLS
TRACER TECHNIQUES
TUBULES
VERTEBRATES