Validation of doubly labeled water method using a ruminant
CO/sub 2/ production (CDP, ml CO/sub 2/ . g-1 . h-1) by captive caribou and reindeer (Rangifer tarandus) was measured using the doubly labeled water method (/sup 3/H/sub 2/O and H2(18)O) and compared with CO/sub 2/ expiration rates (VCO/sub 2/), adjusted for CO/sub 2/ losses in CH4 and urine, as determined by open-circuit respirometry. CDP calculated from samples of blood or urine from a reindeer in winter was 1-3% higher than the adjusted VCO/sub 2/. Differences between values derived by the two methods of 5-20% were found in summer trials with caribou. None of these differences were statistically significant (P greater than 0.05). Differences in summer could in part be explained by the net deposition of /sup 3/H, 18O, and unlabeled CO/sub 2/ in antlers and other growing tissues. Total body water volumes calculated from /sup 3/H/sub 2/O dilution were up to 15% higher than those calculated from H/sub 2/(18)O dilution. The doubly labeled water method appears to be a reasonably accurate method for measuring CDP by caribou and reindeer in winter when growth rates are low, but the method may overestimate CDP by rapidly growing and/or fattening animals.
- OSTI ID:
- 5017283
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RUMINANTS
RESPIRATION
WATER
BODY COMPOSITION
BASAL METABOLISM
CARBON DIOXIDE
DOUBLE LABELLING
MEASURING METHODS
OXYGEN 18
SEASONAL VARIATIONS
TRITIUM COMPOUNDS
ANIMALS
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
EVEN-EVEN NUCLEI
HYDROGEN COMPOUNDS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MAMMALS
METABOLISM
NUCLEI
OXIDES
OXYGEN COMPOUNDS
OXYGEN ISOTOPES
STABLE ISOTOPES
VARIATIONS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques