RNA-binding domain of the A protein component of the U1 small nuclear ribonucleoprotein analyzed by NMR spectroscopy is structurally similar to ribosomal proteins
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
- Duke Univ. Medical Center, Durham, NC (United States)
An RNA recognition motif (RRM) of {approximately}80 amino acids constitutes the core of RNA-binding domains found in a large family of proteins involved in RNA processing. The U1 RNA-binding domain of the A protein component of the human U1 small nuclear ribonucleoprotein (RNP), which encompasses the RRM sequence, was analyzed by using NMR spectroscopy. The domain of the A protein is a highly stable monomer in solution consisting of four antiparallel {beta}-strands and two {alpha}-helices. The highly conserved RNP1 and RNP2 consensus sequences, containing residues previously suggested to be involved in nucleic acid binding, are juxtaposed in adjacent {beta}-strands. Conserved aromatic side chains that are critical for RNA binding are clustered on the surface to the molecule adjacent to a variable loop that influences recognition of specific RNA sequences. The secondary structure and topology of the RRM are similar to those of ribosomal proteins L12 and L30, suggesting a distant evolutionary relationship between these two types of RNA-associated proteins.
- OSTI ID:
- 5015800
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:6; ISSN PNASA; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
CELL CONSTITUENTS
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PROTEINS
RESONANCE
RIBOSOMAL RNA
RIBOSOMES
RNA
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BIOLOGICAL EVOLUTION
CELL CONSTITUENTS
MAGNETIC RESONANCE
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PROTEINS
RESONANCE
RIBOSOMAL RNA
RIBOSOMES
RNA