Whole body and tissue cholesterol turnover in the baboon

Journal Article · Fri Mar 01 00:00:00 EST 1985 · J. Lipid Res.; (United States)

OSTI ID:5014907

Dell, R B; Mott, G E; Jackson, E M; Ramakrishnan, R; Carey, K D; McGill, Jr, H C; Goodman, D S

Cholesterol turnover was studied in four baboons by injecting (/sup 14/C)cholesterol 186 days and (/sup 3/H)cholesterol 4 days before necropsy, and fitting a two- or three-pool model to the resulting specific activity-time data. At necropsy, cholesterol mass and specific activity were determined for the total body and for many tissues. The principal aim of this study was to estimate the extent of cholesterol synthesis in the side pools of the model, by computing the amount of side pool synthesis needed to equal the measured total body cholesterol. Central pool synthesis varied from 61 to 89% of the total cholesterol production rate. Moreover, the finding that the measured total body cholesterol fell within the range obtained from the kinetic analysis by using reasonable assumptions, provides evidence for the physiological validity of the model. A second aim of this study was to explore cholesterol turnover in various tissues. A pool model predicts that rapidly turning over tissues will have higher specific activities at early times and lower specific activities at later times after injection of tracer relative to slowly turning over tissues, except where significant synthesis occurs. Results in all four baboons were similar. Turnover rates for the different tissues loosely fell into three groups which were turning over at fast, intermediate, and slow rates. Finally, the magnitude of variation of cholesterol specific activity was moderate for several distributed tissues (fat, muscle, arteries, and the alimentary tract), but was small for liver. Cholesterol turnover in serial biopsies of skin, muscle, and fat could, however, be fitted with a single pool to estimate tissue turnover rates.

• Research Organization: Columbia Univ. College of Physicians and Surgeons, New York, NY
• OSTI ID: 5014907
• Journal Information: J. Lipid Res.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBON 14 COMPOUNDS
BIOSYNTHESIS
CHOLESTEROL
RADIONUCLIDE KINETICS
MATHEMATICAL MODELS
TRITIUM COMPOUNDS
BIOPSY
DIAGNOSTIC TECHNIQUES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
STEROIDS
STEROLS
SYNTHESIS
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)