Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Pathogenesis of Shigella diarrhea: rabbit intestinal cell microvillus membrane binding site for Shigella toxin

Journal Article · · Infect. Immun.; (United States)
OSTI ID:5013845
; ; ; ; ;

This study examined the binding of purified /sup 125/I-labeled shigella toxin to rabbit jejunal microvillus membranes (MVMs). Toxin binding was concentration dependent, saturable, reversible, and specifically inhibited by unlabeled toxin. The calculated number of toxin molecules bound at 4/sup 0/C was 7.9 X 10(10) (3 X 10(10) to 2 X 10(11))/micrograms of MVM protein or 1.2 X 10(6) per enterocyte. Scatchard analysis showed the binding site to be of a single class with an equilibrium association constant, K, of 4.7 X 10(9) M-1 at 4/sup 0/C. Binding was inversely related to the temperature of incubation. A total of 80% of the labeled toxin binding at 4/sup 0/C dissociated from MVM when the temperature was raised to 37/sup 0/C, but reassociated when the temperature was again brought to 4/sup 0/C. There was no structural or functional change of MVM due to toxin as monitored by electron microscopy or assay of MVM sucrase activity. These studies demonstrate a specific binding site for shigella toxin on rabbit MVMs. The physiological relevance of this receptor remains to be determined.

Research Organization:
New England Medical Center, Boston, MA
OSTI ID:
5013845
Journal Information:
Infect. Immun.; (United States), Journal Name: Infect. Immun.; (United States) Vol. 2; ISSN INFIB
Country of Publication:
United States
Language:
English

Similar Records

Specific receptors for epidermal growth factor in rat intestinal microvillus membranes
Journal Article · Mon Feb 29 23:00:00 EST 1988 · American Journal of Physiology; (USA) · OSTI ID:6871344
Action of cholera toxin in the intestinal epithelial cells
Book · Thu Dec 31 23:00:00 EST 1981 · OSTI ID:6072255
Action of cholera toxin in the intestinal epithelial cells
Technical Report · Thu Dec 31 23:00:00 EST 1981 · OSTI ID:6538673

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
ANTIGENS
BACTERIA
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL BONDS
DAYS LIVING RADIOISOTOPES
DIARRHEA
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
GASTROINTESTINAL TRACT
IN VITRO
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PROTEINS
RABBITS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SHIGELLA
SYMPTOMS
TISSUES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VERTEBRATES