Pathogenesis of Shigella diarrhea: rabbit intestinal cell microvillus membrane binding site for Shigella toxin

Journal Article · Fri Aug 01 00:00:00 EDT 1986 · Infect. Immun.; (United States)

OSTI ID:5013845

Fuchs, G; Mobassaleh, M; Donohue-Rolfe, A; Montgomery, R K; Grand, R J; Keusch, G T

This study examined the binding of purified /sup 125/I-labeled shigella toxin to rabbit jejunal microvillus membranes (MVMs). Toxin binding was concentration dependent, saturable, reversible, and specifically inhibited by unlabeled toxin. The calculated number of toxin molecules bound at 4/sup 0/C was 7.9 X 10(10) (3 X 10(10) to 2 X 10(11))/micrograms of MVM protein or 1.2 X 10(6) per enterocyte. Scatchard analysis showed the binding site to be of a single class with an equilibrium association constant, K, of 4.7 X 10(9) M-1 at 4/sup 0/C. Binding was inversely related to the temperature of incubation. A total of 80% of the labeled toxin binding at 4/sup 0/C dissociated from MVM when the temperature was raised to 37/sup 0/C, but reassociated when the temperature was again brought to 4/sup 0/C. There was no structural or functional change of MVM due to toxin as monitored by electron microscopy or assay of MVM sucrase activity. These studies demonstrate a specific binding site for shigella toxin on rabbit MVMs. The physiological relevance of this receptor remains to be determined.

• Research Organization: New England Medical Center, Boston, MA
• OSTI ID: 5013845
• Journal Information: Infect. Immun.; (United States), Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BACTERIAL DISEASES
PATHOGENESIS
TOXINS
RADIORECEPTOR ASSAY
CELL MEMBRANES
CHEMICAL BONDS
DIARRHEA
EPITHELIUM
IN VITRO
INTESTINES
IODINE 125
LABELLED COMPOUNDS
RABBITS
RECEPTORS
SHIGELLA
ANIMAL TISSUES
ANIMALS
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
SYMPTOMS
TISSUES
TOXIC MATERIALS
TRACER TECHNIQUES
VERTEBRATES
550901* - Pathology- Tracer Techniques